problems with winRhizo

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    I�m trying to scan some roots with the program win Rhizo, I�ve seen the protocol in this web and I�ve followed it, trying to measure a wire with known lenght and diameter. I�ve scanned it at many different levels of resolution and different scales of colour (grey levels, colour..) and I obtain different results and only one which match with the real measure. Then, I�ve tried that option with a different wire and again I can�t get the real measure. Can anybody give me some advice?
    Thank you!

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