Fluorescence in situ hybridisation (FISH) – is a technique which involves the hybridisation of a DNA probe to a complementary sequence on chromosomal preparations to quantify sequences on chromosomal DNA. Fluorescence microscopy analysis allows for probes and targets to be visualised.
For a comprehensive review, see:
- Volpil, E.V. and Bridger, J.M. (2008) FISH glossary: an overview of the fluorescence in situ hybridization technique, BioTechniques 45: 385-409 doi 10.2144/000112811